Looking at the file, I think Mostafa is likely correct that you've deleted deformer history on a tissue or perhaps a bone which has deleted the zEmbedder.
To remove Ziva history from a mesh, please use: Ziva > Remove > Tissue, Cloth or Bone.
Looks like there's a few empty transforms here, could be left over from a polyCombine or polySeparate.
I tried capturing the setup using zBuilder but it looks like it needs the zEmbedder for it to function. Do you have an older version of the file which still has the embedder?
Some other notes, if I may. I'd really suggest going through and naming things. Will at least be helpful for mirroring across the setup later.
Your animation also goes from neutral to posed without any runup (i.e. it jumps over one frame). The sim probably won't like you for that 🙂 You'll need a few frames of runup to go from neutral into the first frame of simulation.